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african green monkey kidney vero cells  (ATCC)


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    ATCC african green monkey kidney vero cells
    Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. <t>(C–J)</t> <t>HEK293T</t> cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) <t>Vero</t> cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).
    African Green Monkey Kidney Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 17976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/african+green+monkey+kidney+cells/Vero/pmc13266186-240-9-19
    Average 99 stars, based on 17976 article reviews
    african green monkey kidney vero cells - by Bioz Stars, 2026-09
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    1) Product Images from "RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation"

    Article Title: RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation

    Journal: iScience

    doi: 10.1016/j.isci.2026.116284

    Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).
    Figure Legend Snippet: Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

    Techniques Used: Sequencing, Control, Infection, Plaque Assay, Transfection, Western Blot, Plasmid Preparation, Immunofluorescence, Staining, Microscopy

    Related Articles

    Cell Culture:

    Article Title: Antiviral activity of berbamine dihydrochloride against Chikungunya virus.
    Article Snippet: .. Baby hamster kidney cells (BHK-21, ATCC CCL-10), African green monkey kidney cells (Vero E6, ATCC CRL-1586), and human hepatocarcinoma cells (Huh-7) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Cultilab, Campinas, SP, Brazil, Cat: D0017) supplemented with 10% fetal bovine serum (FBS; Cultilab, Campinas, SP, Brazil, Cat: 6575-500), 1% of stock of penicillin (10,000 IU/mL)/ streptomycin (10 mg/mL) (P/S; Cultilab, Cat: 001/20), and 1% amphotericin B (Cultilab, Cat: 6498). ..

    Modification:

    Article Title: Antiviral activity of berbamine dihydrochloride against Chikungunya virus.
    Article Snippet: .. Baby hamster kidney cells (BHK-21, ATCC CCL-10), African green monkey kidney cells (Vero E6, ATCC CRL-1586), and human hepatocarcinoma cells (Huh-7) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Cultilab, Campinas, SP, Brazil, Cat: D0017) supplemented with 10% fetal bovine serum (FBS; Cultilab, Campinas, SP, Brazil, Cat: 6575-500), 1% of stock of penicillin (10,000 IU/mL)/ streptomycin (10 mg/mL) (P/S; Cultilab, Cat: 001/20), and 1% amphotericin B (Cultilab, Cat: 6498). ..



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    Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. <t>(C–J)</t> <t>HEK293T</t> cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) <t>Vero</t> cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).
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    Image Search Results


    Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

    Journal: iScience

    Article Title: RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation

    doi: 10.1016/j.isci.2026.116284

    Figure Lengend Snippet: Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

    Article Snippet: Human embryonic kidney (HEK293T), human lung adenocarcinoma (A549), and African green monkey kidney (Vero) cells were purchased from the American Type Culture Collection (ATCC), IFNAR-knockout A549 cells were kindly provided by Prof. Genhong Cheng (Guangzhou National Laboratory).

    Techniques: Sequencing, Control, Infection, Plaque Assay, Transfection, Western Blot, Plasmid Preparation, Immunofluorescence, Staining, Microscopy